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cd45ra antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Miltenyi Biotec cd45ra antibody, anti-human, reafinity
    Cd45ra Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45ra/CD45RA+Antibody%2C+anti-human%2C+REAfinity/custom%40130-122-292%4042502392
    Average 93 stars, based on 83 article reviews
    cd45ra antibody, anti-human, reafinity - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinase Polymerase Amplification:

    Article Title:
    Article Snippet: .. Research Resource Identifiers (RRIDs) were obtained from The Antibody Registry (antibodyregistry.org): HLA-DR (Cat# 564040, BD Biosciences RRID:AB_2738558, Clone G46-6, 1:250) CD8 (Cat# 612942, Becton Dickinson, RRID:AB_2870223, Clone RPA-T8, 1:1000) CD27 (Cat# 741833, Becton Dickinson, RRID:AB_2871168, Clone M-T271, 1:500) CD4 (Cat# 612887, Becton Dickinson, RRID:AB_2870176, Clone SK3, 1:250) CD45RA (Cat# 566114, Becton Dickinson, RRID:AB_2739516, Clone HI100, 1:1000) CD57 (Cat# 393304, Biolegend, RRID:AB_2728425, Clone QA17A04, 1:1000) CD62L (Cat# 563808, Becton Dickinson, RRID:AB_2738433, Clone DREG-56, 1:500) TIM3 (Cat# 565566, Becton Dickinson, RRID:AB_2744370, Clone 7D3, 1:1000) CD25 (Cat# 563701, Becton Dickinson, RRID:AB_2744338, Clone M-A251, 1:500) CFSE (Cat# C34554, ThermoFisher, 1:1000) TIGIT (Cat# 46-9500-42, Invitrogen, RRID:AB_10853679, Clone MBSA43, 1:1000) CCR7 (Cat# 353236, Biolegend, RRID:AB_2563640, Clone G043H7, 1:1000) PD-1 (Cat# 561272, Becton Dickinson, RRID:AB_10611585, Clone N/A, 1:1000) LAG3 (Cat# 369304, Biolegend, RRID:AB_2566480, Clone 11C3C65, 1:500) FVS780 (Cat# 565388, Becton Dickinson, 1:1000) Jovi1 (Cat# ANC-101-030, Ancell, lone JOVI-1, 1:1000) Humanized Jovi 1 and variants were in house generated Anti-idiotype for Jovi-1 was in house generated aCD3 (Cat# 317334, Biolegend, RRID:AB_2561452, Clone OKT3, 1:1000) Streptavidin (Cat# 405243, Biolegend, 1:1000) Anti-CD34 (Cat# 343607, Biolegend, RRID:AB_2074356, Clone 561, 1:200) anti TCR alpha/beta Monoclonal Antibody (WT31) PE conjugated (Cat# 12-9955-42, eBioscience, RRID:AB_10596819, Clone WT31, 1:1000) Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 (Cat# A-21445, Invitrogen, RRID:AB_2535862, Clone N/A, 1:1000) Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Cat# A-21131, Invitrogen, RRID:AB_2535771, Clone N/A, 1:1000) HuAnti-CD34 (Cat# FAB7227A, R&D System, RRID:AB_10972777, Clone QBend10, 1:200) Anti CD3 (Milteny, Cat# 130-113-142, RRID:AB_2725970, Clone REA613, 1:500) Anti CD45RA (Mylteny, Cat# 130-117-747, RRID:AB_2733126, Clone REA1047, 1:500) Anti CCR7 (Miltenyi, Cat# 130-119-583, RRID:AB_2751741, Clone REA546, 1:500) Anti CXCR5 (Miltenyi, Cat# 130-122-795, RRID:AB_2811386, Clone REA103, 1:500) Anti TIM-3 (Miltenyi, Cat# 130-121-334, RRID:AB_2784165, Clone REA635, 1:500) Validation The antibodies used in this study are commercially available and were used for the applications validated by manufacturers. ..

    Article Title:
    Article Snippet: If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies for in vitro treatments: vedolizumab (Takeda), natalizumab (Biogen), ETZ-s (BioXcell [#BE0062]), IgG from rat serum (Merck [I4131]), CD103 (Biolegend [350202]), Purified Mouse IgG1, κ Isotype Ctrl Antibody [400102]). .. Flow cytometry: CD3 (BUV395, SK7, BD Biosciences [564001] / APC, HIT3a, Biolegend [300312] / FITC, OKT3, Biolegend [317306], PE/Cy7, SK7, Biolegend [344816]), CD4 (BUV496, SK3, BD Biosciences [612937] / VioBlue /APC-Vio770, VIT4, Miltenyi Biotec [130-113-781/130-113-211]), CD8a (PerCP/Cy5.5 / FITC, RPA-T8, Biolegend [301032/301006]), CD25 (FITC, M-A251, Biolegend [356106]), CD45RA (VioGreen, REA1047, Miltenyi Biotec [130-117-744]/ APC/Cy7, HI100, Biolegend [304128]), CD69 (APC/Cy7, FN50, Biolegend [310914]), CD103 (APC / PE/Cy7, Ber-ACT8, Biolegend [350216/350212]), CCR9 (PE/Cy7, L053E8, Biolegend [358910]), TCRVα7.2 (BV421, OF5A12, BD Biosciences [749494]), α4 (FITC, MZ18-24A9, Miltenyi Biotec [130-122-933] / PE/Cy7, 9F10, Biolegend [304313]), β1 (AF488 / PE, TS2/16, Biolegend [303003/303016]), β7 (BV605, FIB504, BD Biosciences [564284] / PE, FIB27, Biolegend [121006]), GNLY (PE, DH2, Biolegend [348004]), GZMB (PacificBlue, GB11, Biolegend [515408]), GZMK (APC, GM26E7, Biolegend [370510]), IFNγ (PE/Cy7, B27, Biolegend [506528]), IL2 (BV421, 5344.111, BD Biosciences [562914]), IL17A (PE, BL168, Biolegend [512306]), TNFα (PE, Mab11, Biolegend [502909]) Validation All commercially obtained antibodies were previously validated by the manufacturer for the specific application. ..

    Generated:

    Article Title:
    Article Snippet: .. Research Resource Identifiers (RRIDs) were obtained from The Antibody Registry (antibodyregistry.org): HLA-DR (Cat# 564040, BD Biosciences RRID:AB_2738558, Clone G46-6, 1:250) CD8 (Cat# 612942, Becton Dickinson, RRID:AB_2870223, Clone RPA-T8, 1:1000) CD27 (Cat# 741833, Becton Dickinson, RRID:AB_2871168, Clone M-T271, 1:500) CD4 (Cat# 612887, Becton Dickinson, RRID:AB_2870176, Clone SK3, 1:250) CD45RA (Cat# 566114, Becton Dickinson, RRID:AB_2739516, Clone HI100, 1:1000) CD57 (Cat# 393304, Biolegend, RRID:AB_2728425, Clone QA17A04, 1:1000) CD62L (Cat# 563808, Becton Dickinson, RRID:AB_2738433, Clone DREG-56, 1:500) TIM3 (Cat# 565566, Becton Dickinson, RRID:AB_2744370, Clone 7D3, 1:1000) CD25 (Cat# 563701, Becton Dickinson, RRID:AB_2744338, Clone M-A251, 1:500) CFSE (Cat# C34554, ThermoFisher, 1:1000) TIGIT (Cat# 46-9500-42, Invitrogen, RRID:AB_10853679, Clone MBSA43, 1:1000) CCR7 (Cat# 353236, Biolegend, RRID:AB_2563640, Clone G043H7, 1:1000) PD-1 (Cat# 561272, Becton Dickinson, RRID:AB_10611585, Clone N/A, 1:1000) LAG3 (Cat# 369304, Biolegend, RRID:AB_2566480, Clone 11C3C65, 1:500) FVS780 (Cat# 565388, Becton Dickinson, 1:1000) Jovi1 (Cat# ANC-101-030, Ancell, lone JOVI-1, 1:1000) Humanized Jovi 1 and variants were in house generated Anti-idiotype for Jovi-1 was in house generated aCD3 (Cat# 317334, Biolegend, RRID:AB_2561452, Clone OKT3, 1:1000) Streptavidin (Cat# 405243, Biolegend, 1:1000) Anti-CD34 (Cat# 343607, Biolegend, RRID:AB_2074356, Clone 561, 1:200) anti TCR alpha/beta Monoclonal Antibody (WT31) PE conjugated (Cat# 12-9955-42, eBioscience, RRID:AB_10596819, Clone WT31, 1:1000) Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 (Cat# A-21445, Invitrogen, RRID:AB_2535862, Clone N/A, 1:1000) Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Cat# A-21131, Invitrogen, RRID:AB_2535771, Clone N/A, 1:1000) HuAnti-CD34 (Cat# FAB7227A, R&D System, RRID:AB_10972777, Clone QBend10, 1:200) Anti CD3 (Milteny, Cat# 130-113-142, RRID:AB_2725970, Clone REA613, 1:500) Anti CD45RA (Mylteny, Cat# 130-117-747, RRID:AB_2733126, Clone REA1047, 1:500) Anti CCR7 (Miltenyi, Cat# 130-119-583, RRID:AB_2751741, Clone REA546, 1:500) Anti CXCR5 (Miltenyi, Cat# 130-122-795, RRID:AB_2811386, Clone REA103, 1:500) Anti TIM-3 (Miltenyi, Cat# 130-121-334, RRID:AB_2784165, Clone REA635, 1:500) Validation The antibodies used in this study are commercially available and were used for the applications validated by manufacturers. ..

    Biomarker Discovery:

    Article Title:
    Article Snippet: .. Research Resource Identifiers (RRIDs) were obtained from The Antibody Registry (antibodyregistry.org): HLA-DR (Cat# 564040, BD Biosciences RRID:AB_2738558, Clone G46-6, 1:250) CD8 (Cat# 612942, Becton Dickinson, RRID:AB_2870223, Clone RPA-T8, 1:1000) CD27 (Cat# 741833, Becton Dickinson, RRID:AB_2871168, Clone M-T271, 1:500) CD4 (Cat# 612887, Becton Dickinson, RRID:AB_2870176, Clone SK3, 1:250) CD45RA (Cat# 566114, Becton Dickinson, RRID:AB_2739516, Clone HI100, 1:1000) CD57 (Cat# 393304, Biolegend, RRID:AB_2728425, Clone QA17A04, 1:1000) CD62L (Cat# 563808, Becton Dickinson, RRID:AB_2738433, Clone DREG-56, 1:500) TIM3 (Cat# 565566, Becton Dickinson, RRID:AB_2744370, Clone 7D3, 1:1000) CD25 (Cat# 563701, Becton Dickinson, RRID:AB_2744338, Clone M-A251, 1:500) CFSE (Cat# C34554, ThermoFisher, 1:1000) TIGIT (Cat# 46-9500-42, Invitrogen, RRID:AB_10853679, Clone MBSA43, 1:1000) CCR7 (Cat# 353236, Biolegend, RRID:AB_2563640, Clone G043H7, 1:1000) PD-1 (Cat# 561272, Becton Dickinson, RRID:AB_10611585, Clone N/A, 1:1000) LAG3 (Cat# 369304, Biolegend, RRID:AB_2566480, Clone 11C3C65, 1:500) FVS780 (Cat# 565388, Becton Dickinson, 1:1000) Jovi1 (Cat# ANC-101-030, Ancell, lone JOVI-1, 1:1000) Humanized Jovi 1 and variants were in house generated Anti-idiotype for Jovi-1 was in house generated aCD3 (Cat# 317334, Biolegend, RRID:AB_2561452, Clone OKT3, 1:1000) Streptavidin (Cat# 405243, Biolegend, 1:1000) Anti-CD34 (Cat# 343607, Biolegend, RRID:AB_2074356, Clone 561, 1:200) anti TCR alpha/beta Monoclonal Antibody (WT31) PE conjugated (Cat# 12-9955-42, eBioscience, RRID:AB_10596819, Clone WT31, 1:1000) Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 (Cat# A-21445, Invitrogen, RRID:AB_2535862, Clone N/A, 1:1000) Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (Cat# A-21131, Invitrogen, RRID:AB_2535771, Clone N/A, 1:1000) HuAnti-CD34 (Cat# FAB7227A, R&D System, RRID:AB_10972777, Clone QBend10, 1:200) Anti CD3 (Milteny, Cat# 130-113-142, RRID:AB_2725970, Clone REA613, 1:500) Anti CD45RA (Mylteny, Cat# 130-117-747, RRID:AB_2733126, Clone REA1047, 1:500) Anti CCR7 (Miltenyi, Cat# 130-119-583, RRID:AB_2751741, Clone REA546, 1:500) Anti CXCR5 (Miltenyi, Cat# 130-122-795, RRID:AB_2811386, Clone REA103, 1:500) Anti TIM-3 (Miltenyi, Cat# 130-121-334, RRID:AB_2784165, Clone REA635, 1:500) Validation The antibodies used in this study are commercially available and were used for the applications validated by manufacturers. ..

    Article Title:
    Article Snippet: If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies for in vitro treatments: vedolizumab (Takeda), natalizumab (Biogen), ETZ-s (BioXcell [#BE0062]), IgG from rat serum (Merck [I4131]), CD103 (Biolegend [350202]), Purified Mouse IgG1, κ Isotype Ctrl Antibody [400102]). .. Flow cytometry: CD3 (BUV395, SK7, BD Biosciences [564001] / APC, HIT3a, Biolegend [300312] / FITC, OKT3, Biolegend [317306], PE/Cy7, SK7, Biolegend [344816]), CD4 (BUV496, SK3, BD Biosciences [612937] / VioBlue /APC-Vio770, VIT4, Miltenyi Biotec [130-113-781/130-113-211]), CD8a (PerCP/Cy5.5 / FITC, RPA-T8, Biolegend [301032/301006]), CD25 (FITC, M-A251, Biolegend [356106]), CD45RA (VioGreen, REA1047, Miltenyi Biotec [130-117-744]/ APC/Cy7, HI100, Biolegend [304128]), CD69 (APC/Cy7, FN50, Biolegend [310914]), CD103 (APC / PE/Cy7, Ber-ACT8, Biolegend [350216/350212]), CCR9 (PE/Cy7, L053E8, Biolegend [358910]), TCRVα7.2 (BV421, OF5A12, BD Biosciences [749494]), α4 (FITC, MZ18-24A9, Miltenyi Biotec [130-122-933] / PE/Cy7, 9F10, Biolegend [304313]), β1 (AF488 / PE, TS2/16, Biolegend [303003/303016]), β7 (BV605, FIB504, BD Biosciences [564284] / PE, FIB27, Biolegend [121006]), GNLY (PE, DH2, Biolegend [348004]), GZMB (PacificBlue, GB11, Biolegend [515408]), GZMK (APC, GM26E7, Biolegend [370510]), IFNγ (PE/Cy7, B27, Biolegend [506528]), IL2 (BV421, 5344.111, BD Biosciences [562914]), IL17A (PE, BL168, Biolegend [512306]), TNFα (PE, Mab11, Biolegend [502909]) Validation All commercially obtained antibodies were previously validated by the manufacturer for the specific application. ..

    Marker:

    Article Title: Targeting CD276 with Adapter-CAR T-cells provides a novel therapeutic strategy in small cell lung cancer and prevents CD276-dependent fratricide
    Article Snippet: .. T-cell subtypes were analysed with CD45RA (APC; Miltenyi Biotec) and CD62L (FITC, Miltenyi Biotec) marker expression. ..

    Article Title: Method for improving production of car T cells
    Article Snippet: .. Marker Surface Detection The proportion of T-cells expressing the CAR at their surface was then quantified using the following antibodies: CD3: Clone BW264/56, Vioblue (Miltenyi #130-094-363), TCRαβ: Clone REA652, PE (Miltenyi #130-109-920), CD4: Clone VIT4, PEVio770 (Miltenyi #130-096-552), CD8: Clone SK1, BV510 (Biolegend #344732), CD62L: Clone 145/15, APC (Miltenyi #130-113-617), CD45RA: Clone T6D11, Vioblue (Miltenyi #130-113-360), PD1: Clone REA1165, PE (Miltenyi #130-120-388) and LAG3: Clone 11C3C65, BV421 (Biolegend #369313). .. Antigen Dependent Proliferation Raji cells were treated with 20 Gy using a CellRad X-ray irradiation system (Faxitron, Tucson, AZ, USA), washed twice, counted and 500,000 were plated with 500,000 T-cells (1:1) in duplicate into 1 ml final volume of X-VivoTM 15 media with 5% hAB serum, but lacking IL-2, in a 24 well plate.

    Expressing:

    Article Title: Targeting CD276 with Adapter-CAR T-cells provides a novel therapeutic strategy in small cell lung cancer and prevents CD276-dependent fratricide
    Article Snippet: .. T-cell subtypes were analysed with CD45RA (APC; Miltenyi Biotec) and CD62L (FITC, Miltenyi Biotec) marker expression. ..

    Article Title: Method for improving production of car T cells
    Article Snippet: .. Marker Surface Detection The proportion of T-cells expressing the CAR at their surface was then quantified using the following antibodies: CD3: Clone BW264/56, Vioblue (Miltenyi #130-094-363), TCRαβ: Clone REA652, PE (Miltenyi #130-109-920), CD4: Clone VIT4, PEVio770 (Miltenyi #130-096-552), CD8: Clone SK1, BV510 (Biolegend #344732), CD62L: Clone 145/15, APC (Miltenyi #130-113-617), CD45RA: Clone T6D11, Vioblue (Miltenyi #130-113-360), PD1: Clone REA1165, PE (Miltenyi #130-120-388) and LAG3: Clone 11C3C65, BV421 (Biolegend #369313). .. Antigen Dependent Proliferation Raji cells were treated with 20 Gy using a CellRad X-ray irradiation system (Faxitron, Tucson, AZ, USA), washed twice, counted and 500,000 were plated with 500,000 T-cells (1:1) in duplicate into 1 ml final volume of X-VivoTM 15 media with 5% hAB serum, but lacking IL-2, in a 24 well plate.

    Staining:

    Article Title: Frequency of synaptic antigen-specific CD4 + T cells in dementia is age-dependent but not correlated with cognitive impairment.
    Article Snippet: .. Cell staining and Fluorescence activated cell scanning (FACS) Extracellular markers were stained with antibodies targeting CD14 (FITC, Miltenyi, TÜK4, 130–113–146, 1:50), CD20 (FITC, Miltenyi, LT20, 130–113–373, 1:50), CD4 (VIT4, VioBlue, Miltenyi, 130–113–219, 1:50), CD45RA (VioGreen, Miltenyi, REA562, 130–113–931, 1:50) and CCR7 (APC, Miltenyi, REA546, 130–120– 466, 1:50). .. Furthermore, a viability staining was applied (488/520 FITC, Miltenyi, 130–109–812).

    Fluorescence:

    Article Title: Frequency of synaptic antigen-specific CD4 + T cells in dementia is age-dependent but not correlated with cognitive impairment.
    Article Snippet: .. Cell staining and Fluorescence activated cell scanning (FACS) Extracellular markers were stained with antibodies targeting CD14 (FITC, Miltenyi, TÜK4, 130–113–146, 1:50), CD20 (FITC, Miltenyi, LT20, 130–113–373, 1:50), CD4 (VIT4, VioBlue, Miltenyi, 130–113–219, 1:50), CD45RA (VioGreen, Miltenyi, REA562, 130–113–931, 1:50) and CCR7 (APC, Miltenyi, REA546, 130–120– 466, 1:50). .. Furthermore, a viability staining was applied (488/520 FITC, Miltenyi, 130–109–812).

    FACS:

    Article Title: Frequency of synaptic antigen-specific CD4 + T cells in dementia is age-dependent but not correlated with cognitive impairment.
    Article Snippet: .. Cell staining and Fluorescence activated cell scanning (FACS) Extracellular markers were stained with antibodies targeting CD14 (FITC, Miltenyi, TÜK4, 130–113–146, 1:50), CD20 (FITC, Miltenyi, LT20, 130–113–373, 1:50), CD4 (VIT4, VioBlue, Miltenyi, 130–113–219, 1:50), CD45RA (VioGreen, Miltenyi, REA562, 130–113–931, 1:50) and CCR7 (APC, Miltenyi, REA546, 130–120– 466, 1:50). .. Furthermore, a viability staining was applied (488/520 FITC, Miltenyi, 130–109–812).

    Imaging:

    Article Title: Inhibition of autophagy enhances the antitumor efficacy of T/CAR T cell against neuroblastoma
    Article Snippet: Fluorochromes were photobleached or released using REAlease technology (Miltenyi) after image acquisition at the end of each staining cycle. .. The following antibodies (all from Miltenyi Biotec) were used: Ki67, CD3, CD4, CD8, CD44, CD45RA, CD45RO, CD57, CD183, CD196, LAG3 (CD223), PD-1 (CD279), TIM3 (CD366), FoxP3, HLA-DR. Acquired images were processed and analyzed by MACSiQ ® View Imaging Software (Miltenyi Biotec) following current processing work-flow (Miltenyi Biotec). ..

    Software:

    Article Title: Inhibition of autophagy enhances the antitumor efficacy of T/CAR T cell against neuroblastoma
    Article Snippet: Fluorochromes were photobleached or released using REAlease technology (Miltenyi) after image acquisition at the end of each staining cycle. .. The following antibodies (all from Miltenyi Biotec) were used: Ki67, CD3, CD4, CD8, CD44, CD45RA, CD45RO, CD57, CD183, CD196, LAG3 (CD223), PD-1 (CD279), TIM3 (CD366), FoxP3, HLA-DR. Acquired images were processed and analyzed by MACSiQ ® View Imaging Software (Miltenyi Biotec) following current processing work-flow (Miltenyi Biotec). ..

    Flow Cytometry:

    Article Title:
    Article Snippet: If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies for in vitro treatments: vedolizumab (Takeda), natalizumab (Biogen), ETZ-s (BioXcell [#BE0062]), IgG from rat serum (Merck [I4131]), CD103 (Biolegend [350202]), Purified Mouse IgG1, κ Isotype Ctrl Antibody [400102]). .. Flow cytometry: CD3 (BUV395, SK7, BD Biosciences [564001] / APC, HIT3a, Biolegend [300312] / FITC, OKT3, Biolegend [317306], PE/Cy7, SK7, Biolegend [344816]), CD4 (BUV496, SK3, BD Biosciences [612937] / VioBlue /APC-Vio770, VIT4, Miltenyi Biotec [130-113-781/130-113-211]), CD8a (PerCP/Cy5.5 / FITC, RPA-T8, Biolegend [301032/301006]), CD25 (FITC, M-A251, Biolegend [356106]), CD45RA (VioGreen, REA1047, Miltenyi Biotec [130-117-744]/ APC/Cy7, HI100, Biolegend [304128]), CD69 (APC/Cy7, FN50, Biolegend [310914]), CD103 (APC / PE/Cy7, Ber-ACT8, Biolegend [350216/350212]), CCR9 (PE/Cy7, L053E8, Biolegend [358910]), TCRVα7.2 (BV421, OF5A12, BD Biosciences [749494]), α4 (FITC, MZ18-24A9, Miltenyi Biotec [130-122-933] / PE/Cy7, 9F10, Biolegend [304313]), β1 (AF488 / PE, TS2/16, Biolegend [303003/303016]), β7 (BV605, FIB504, BD Biosciences [564284] / PE, FIB27, Biolegend [121006]), GNLY (PE, DH2, Biolegend [348004]), GZMB (PacificBlue, GB11, Biolegend [515408]), GZMK (APC, GM26E7, Biolegend [370510]), IFNγ (PE/Cy7, B27, Biolegend [506528]), IL2 (BV421, 5344.111, BD Biosciences [562914]), IL17A (PE, BL168, Biolegend [512306]), TNFα (PE, Mab11, Biolegend [502909]) Validation All commercially obtained antibodies were previously validated by the manufacturer for the specific application. ..



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    ( A ) Representative western blots (top) and densitometric quantification (bottom) of Stk26 protein levels within peripheral LNs CD4 T cells isolated from female C57BL/6J mice on different diet groups for 14 weeks (groups 1–3 in Fig. ). Data are presented as mean +/− SD ( n = 3 mice per group). Unpaired nonparametric Mann–Whitney T test; n.s. ( B ) Representative flow cytometry histograms (left) showing LC3II expression in splenocytes from the three 14-week mouse groups (groups 1–3 in Fig. ). Splenocytes were cultured ex vivo in the presence of 100 μM of chloroquine within RPMI for 4 h. Scatter plots (right) show autophagy flux, calculated as the difference in mean fluorescence intensity (MFI) of LC3II between chloroquine-treated and vehicle control groups. Analysis was gated on live (near IR-) CD4 + CD44 + CXCR3+ or CD4 + CD44 + LFA1 + T cell populations. Data are from one experiment ( n = 6 female C57BL/6J mice per group). Results are shown as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), ** P < 0.01. ( C ) Representative flow cytometry plots (left) of CD4 + KLRG1+ cells gated within the live (near IR-) CD4 T cell population from peripheral LNs of female C57BL/6J mice on a 14-week CD or HFD-RE. Scatter plots (right) show the percentage of CD4 + KLRG1+ cells within the CD4 + CD44+ or CD4 + LFA1+ populations. Data is from one experiment ( n = 5–6 mice per group) and is presented as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), * P < 0.05. ( D ) Scatter plots showing CD4 and CD8 <t>(CD45RA</t> + CCR7-) human TEMRA from PBMCs of control ( n = 10) and individuals with Alstrom syndrome ( n = 10). Data are presented as mean +/− SD. Paired two-tailed Wilcoxon T test; * P < 0.05. ( E ) Scatter plots showing the percentage of human CD4 TEMRA (CCR7-CD45RO-) cells from PBMCs from individuals ( n = 13) before and after semaglutide treatment. All samples were gated on live (near IR-) populations. Paired nonparametric Wilcoxon T test; n.s. ( F ) Flow cytometry analysis of human CD4 + TEMRA cells (CD3 + CD4 + CD45RA + CD27 − ) in subcutaneous abdominal adipose tissue and lysed whole blood. In adipose, data are expressed as a percentage of CD4 + T cells. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention= 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s. ( G ) Heatmap displaying transcriptomic data of selected genes with previous implications with autophagy and/or senescence from murine CD44+ memory T cells isolated from spleen of female C57BL/6J mice on different diet groups for 14-week ( n = 3 female C57BL/6J mice per group; groups 1–3 in Fig. ). The analysis was performed using the Nanostring Mouse PanCancer Immune Profiling panel, covering 770 genes including genes related to autophagy and/or senescence. .
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    ( A ) Representative western blots (top) and densitometric quantification (bottom) of Stk26 protein levels within peripheral LNs CD4 T cells isolated from female C57BL/6J mice on different diet groups for 14 weeks (groups 1–3 in Fig. ). Data are presented as mean +/− SD ( n = 3 mice per group). Unpaired nonparametric Mann–Whitney T test; n.s. ( B ) Representative flow cytometry histograms (left) showing LC3II expression in splenocytes from the three 14-week mouse groups (groups 1–3 in Fig. ). Splenocytes were cultured ex vivo in the presence of 100 μM of chloroquine within RPMI for 4 h. Scatter plots (right) show autophagy flux, calculated as the difference in mean fluorescence intensity (MFI) of LC3II between chloroquine-treated and vehicle control groups. Analysis was gated on live (near IR-) CD4 + CD44 + CXCR3+ or CD4 + CD44 + LFA1 + T cell populations. Data are from one experiment ( n = 6 female C57BL/6J mice per group). Results are shown as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), ** P < 0.01. ( C ) Representative flow cytometry plots (left) of CD4 + KLRG1+ cells gated within the live (near IR-) CD4 T cell population from peripheral LNs of female C57BL/6J mice on a 14-week CD or HFD-RE. Scatter plots (right) show the percentage of CD4 + KLRG1+ cells within the CD4 + CD44+ or CD4 + LFA1+ populations. Data is from one experiment ( n = 5–6 mice per group) and is presented as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), * P < 0.05. ( D ) Scatter plots showing CD4 and CD8 <t>(CD45RA</t> + CCR7-) human TEMRA from PBMCs of control ( n = 10) and individuals with Alstrom syndrome ( n = 10). Data are presented as mean +/− SD. Paired two-tailed Wilcoxon T test; * P < 0.05. ( E ) Scatter plots showing the percentage of human CD4 TEMRA (CCR7-CD45RO-) cells from PBMCs from individuals ( n = 13) before and after semaglutide treatment. All samples were gated on live (near IR-) populations. Paired nonparametric Wilcoxon T test; n.s. ( F ) Flow cytometry analysis of human CD4 + TEMRA cells (CD3 + CD4 + CD45RA + CD27 − ) in subcutaneous abdominal adipose tissue and lysed whole blood. In adipose, data are expressed as a percentage of CD4 + T cells. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention= 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s. ( G ) Heatmap displaying transcriptomic data of selected genes with previous implications with autophagy and/or senescence from murine CD44+ memory T cells isolated from spleen of female C57BL/6J mice on different diet groups for 14-week ( n = 3 female C57BL/6J mice per group; groups 1–3 in Fig. ). The analysis was performed using the Nanostring Mouse PanCancer Immune Profiling panel, covering 770 genes including genes related to autophagy and/or senescence. .
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    ( A ) Representative western blots (top) and densitometric quantification (bottom) of Stk26 protein levels within peripheral LNs CD4 T cells isolated from female C57BL/6J mice on different diet groups for 14 weeks (groups 1–3 in Fig. ). Data are presented as mean +/− SD ( n = 3 mice per group). Unpaired nonparametric Mann–Whitney T test; n.s. ( B ) Representative flow cytometry histograms (left) showing LC3II expression in splenocytes from the three 14-week mouse groups (groups 1–3 in Fig. ). Splenocytes were cultured ex vivo in the presence of 100 μM of chloroquine within RPMI for 4 h. Scatter plots (right) show autophagy flux, calculated as the difference in mean fluorescence intensity (MFI) of LC3II between chloroquine-treated and vehicle control groups. Analysis was gated on live (near IR-) CD4 + CD44 + CXCR3+ or CD4 + CD44 + LFA1 + T cell populations. Data are from one experiment ( n = 6 female C57BL/6J mice per group). Results are shown as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), ** P < 0.01. ( C ) Representative flow cytometry plots (left) of CD4 + KLRG1+ cells gated within the live (near IR-) CD4 T cell population from peripheral LNs of female C57BL/6J mice on a 14-week CD or HFD-RE. Scatter plots (right) show the percentage of CD4 + KLRG1+ cells within the CD4 + CD44+ or CD4 + LFA1+ populations. Data is from one experiment ( n = 5–6 mice per group) and is presented as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), * P < 0.05. ( D ) Scatter plots showing CD4 and CD8 <t>(CD45RA</t> + CCR7-) human TEMRA from PBMCs of control ( n = 10) and individuals with Alstrom syndrome ( n = 10). Data are presented as mean +/− SD. Paired two-tailed Wilcoxon T test; * P < 0.05. ( E ) Scatter plots showing the percentage of human CD4 TEMRA (CCR7-CD45RO-) cells from PBMCs from individuals ( n = 13) before and after semaglutide treatment. All samples were gated on live (near IR-) populations. Paired nonparametric Wilcoxon T test; n.s. ( F ) Flow cytometry analysis of human CD4 + TEMRA cells (CD3 + CD4 + CD45RA + CD27 − ) in subcutaneous abdominal adipose tissue and lysed whole blood. In adipose, data are expressed as a percentage of CD4 + T cells. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention= 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s. ( G ) Heatmap displaying transcriptomic data of selected genes with previous implications with autophagy and/or senescence from murine CD44+ memory T cells isolated from spleen of female C57BL/6J mice on different diet groups for 14-week ( n = 3 female C57BL/6J mice per group; groups 1–3 in Fig. ). The analysis was performed using the Nanostring Mouse PanCancer Immune Profiling panel, covering 770 genes including genes related to autophagy and/or senescence. .
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    ( A ) Representative western blots (top) and densitometric quantification (bottom) of Stk26 protein levels within peripheral LNs CD4 T cells isolated from female C57BL/6J mice on different diet groups for 14 weeks (groups 1–3 in Fig. ). Data are presented as mean +/− SD ( n = 3 mice per group). Unpaired nonparametric Mann–Whitney T test; n.s. ( B ) Representative flow cytometry histograms (left) showing LC3II expression in splenocytes from the three 14-week mouse groups (groups 1–3 in Fig. ). Splenocytes were cultured ex vivo in the presence of 100 μM of chloroquine within RPMI for 4 h. Scatter plots (right) show autophagy flux, calculated as the difference in mean fluorescence intensity (MFI) of LC3II between chloroquine-treated and vehicle control groups. Analysis was gated on live (near IR-) CD4 + CD44 + CXCR3+ or CD4 + CD44 + LFA1 + T cell populations. Data are from one experiment ( n = 6 female C57BL/6J mice per group). Results are shown as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), ** P < 0.01. ( C ) Representative flow cytometry plots (left) of CD4 + KLRG1+ cells gated within the live (near IR-) CD4 T cell population from peripheral LNs of female C57BL/6J mice on a 14-week CD or HFD-RE. Scatter plots (right) show the percentage of CD4 + KLRG1+ cells within the CD4 + CD44+ or CD4 + LFA1+ populations. Data is from one experiment ( n = 5–6 mice per group) and is presented as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), * P < 0.05. ( D ) Scatter plots showing CD4 and CD8 <t>(CD45RA</t> + CCR7-) human TEMRA from PBMCs of control ( n = 10) and individuals with Alstrom syndrome ( n = 10). Data are presented as mean +/− SD. Paired two-tailed Wilcoxon T test; * P < 0.05. ( E ) Scatter plots showing the percentage of human CD4 TEMRA (CCR7-CD45RO-) cells from PBMCs from individuals ( n = 13) before and after semaglutide treatment. All samples were gated on live (near IR-) populations. Paired nonparametric Wilcoxon T test; n.s. ( F ) Flow cytometry analysis of human CD4 + TEMRA cells (CD3 + CD4 + CD45RA + CD27 − ) in subcutaneous abdominal adipose tissue and lysed whole blood. In adipose, data are expressed as a percentage of CD4 + T cells. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention= 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s. ( G ) Heatmap displaying transcriptomic data of selected genes with previous implications with autophagy and/or senescence from murine CD44+ memory T cells isolated from spleen of female C57BL/6J mice on different diet groups for 14-week ( n = 3 female C57BL/6J mice per group; groups 1–3 in Fig. ). The analysis was performed using the Nanostring Mouse PanCancer Immune Profiling panel, covering 770 genes including genes related to autophagy and/or senescence. .
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    ( A ) Representative western blots (top) and densitometric quantification (bottom) of Stk26 protein levels within peripheral LNs CD4 T cells isolated from female C57BL/6J mice on different diet groups for 14 weeks (groups 1–3 in Fig. ). Data are presented as mean +/− SD ( n = 3 mice per group). Unpaired nonparametric Mann–Whitney T test; n.s. ( B ) Representative flow cytometry histograms (left) showing LC3II expression in splenocytes from the three 14-week mouse groups (groups 1–3 in Fig. ). Splenocytes were cultured ex vivo in the presence of 100 μM of chloroquine within RPMI for 4 h. Scatter plots (right) show autophagy flux, calculated as the difference in mean fluorescence intensity (MFI) of LC3II between chloroquine-treated and vehicle control groups. Analysis was gated on live (near IR-) CD4 + CD44 + CXCR3+ or CD4 + CD44 + LFA1 + T cell populations. Data are from one experiment ( n = 6 female C57BL/6J mice per group). Results are shown as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), ** P < 0.01. ( C ) Representative flow cytometry plots (left) of CD4 + KLRG1+ cells gated within the live (near IR-) CD4 T cell population from peripheral LNs of female C57BL/6J mice on a 14-week CD or HFD-RE. Scatter plots (right) show the percentage of CD4 + KLRG1+ cells within the CD4 + CD44+ or CD4 + LFA1+ populations. Data is from one experiment ( n = 5–6 mice per group) and is presented as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), * P < 0.05. ( D ) Scatter plots showing CD4 and CD8 <t>(CD45RA</t> + CCR7-) human TEMRA from PBMCs of control ( n = 10) and individuals with Alstrom syndrome ( n = 10). Data are presented as mean +/− SD. Paired two-tailed Wilcoxon T test; * P < 0.05. ( E ) Scatter plots showing the percentage of human CD4 TEMRA (CCR7-CD45RO-) cells from PBMCs from individuals ( n = 13) before and after semaglutide treatment. All samples were gated on live (near IR-) populations. Paired nonparametric Wilcoxon T test; n.s. ( F ) Flow cytometry analysis of human CD4 + TEMRA cells (CD3 + CD4 + CD45RA + CD27 − ) in subcutaneous abdominal adipose tissue and lysed whole blood. In adipose, data are expressed as a percentage of CD4 + T cells. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention= 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s. ( G ) Heatmap displaying transcriptomic data of selected genes with previous implications with autophagy and/or senescence from murine CD44+ memory T cells isolated from spleen of female C57BL/6J mice on different diet groups for 14-week ( n = 3 female C57BL/6J mice per group; groups 1–3 in Fig. ). The analysis was performed using the Nanostring Mouse PanCancer Immune Profiling panel, covering 770 genes including genes related to autophagy and/or senescence. .
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    ( A ) Representative western blots (top) and densitometric quantification (bottom) of Stk26 protein levels within peripheral LNs CD4 T cells isolated from female C57BL/6J mice on different diet groups for 14 weeks (groups 1–3 in Fig. ). Data are presented as mean +/− SD ( n = 3 mice per group). Unpaired nonparametric Mann–Whitney T test; n.s. ( B ) Representative flow cytometry histograms (left) showing LC3II expression in splenocytes from the three 14-week mouse groups (groups 1–3 in Fig. ). Splenocytes were cultured ex vivo in the presence of 100 μM of chloroquine within RPMI for 4 h. Scatter plots (right) show autophagy flux, calculated as the difference in mean fluorescence intensity (MFI) of LC3II between chloroquine-treated and vehicle control groups. Analysis was gated on live (near IR-) CD4 + CD44 + CXCR3+ or CD4 + CD44 + LFA1 + T cell populations. Data are from one experiment ( n = 6 female C57BL/6J mice per group). Results are shown as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), ** P < 0.01. ( C ) Representative flow cytometry plots (left) of CD4 + KLRG1+ cells gated within the live (near IR-) CD4 T cell population from peripheral LNs of female C57BL/6J mice on a 14-week CD or HFD-RE. Scatter plots (right) show the percentage of CD4 + KLRG1+ cells within the CD4 + CD44+ or CD4 + LFA1+ populations. Data is from one experiment ( n = 5–6 mice per group) and is presented as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), * P < 0.05. ( D ) Scatter plots showing CD4 and CD8 <t>(CD45RA</t> + CCR7-) human TEMRA from PBMCs of control ( n = 10) and individuals with Alstrom syndrome ( n = 10). Data are presented as mean +/− SD. Paired two-tailed Wilcoxon T test; * P < 0.05. ( E ) Scatter plots showing the percentage of human CD4 TEMRA (CCR7-CD45RO-) cells from PBMCs from individuals ( n = 13) before and after semaglutide treatment. All samples were gated on live (near IR-) populations. Paired nonparametric Wilcoxon T test; n.s. ( F ) Flow cytometry analysis of human CD4 + TEMRA cells (CD3 + CD4 + CD45RA + CD27 − ) in subcutaneous abdominal adipose tissue and lysed whole blood. In adipose, data are expressed as a percentage of CD4 + T cells. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention= 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s. ( G ) Heatmap displaying transcriptomic data of selected genes with previous implications with autophagy and/or senescence from murine CD44+ memory T cells isolated from spleen of female C57BL/6J mice on different diet groups for 14-week ( n = 3 female C57BL/6J mice per group; groups 1–3 in Fig. ). The analysis was performed using the Nanostring Mouse PanCancer Immune Profiling panel, covering 770 genes including genes related to autophagy and/or senescence. .
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    Image Search Results


    ( A ) Representative western blots (top) and densitometric quantification (bottom) of Stk26 protein levels within peripheral LNs CD4 T cells isolated from female C57BL/6J mice on different diet groups for 14 weeks (groups 1–3 in Fig. ). Data are presented as mean +/− SD ( n = 3 mice per group). Unpaired nonparametric Mann–Whitney T test; n.s. ( B ) Representative flow cytometry histograms (left) showing LC3II expression in splenocytes from the three 14-week mouse groups (groups 1–3 in Fig. ). Splenocytes were cultured ex vivo in the presence of 100 μM of chloroquine within RPMI for 4 h. Scatter plots (right) show autophagy flux, calculated as the difference in mean fluorescence intensity (MFI) of LC3II between chloroquine-treated and vehicle control groups. Analysis was gated on live (near IR-) CD4 + CD44 + CXCR3+ or CD4 + CD44 + LFA1 + T cell populations. Data are from one experiment ( n = 6 female C57BL/6J mice per group). Results are shown as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), ** P < 0.01. ( C ) Representative flow cytometry plots (left) of CD4 + KLRG1+ cells gated within the live (near IR-) CD4 T cell population from peripheral LNs of female C57BL/6J mice on a 14-week CD or HFD-RE. Scatter plots (right) show the percentage of CD4 + KLRG1+ cells within the CD4 + CD44+ or CD4 + LFA1+ populations. Data is from one experiment ( n = 5–6 mice per group) and is presented as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), * P < 0.05. ( D ) Scatter plots showing CD4 and CD8 (CD45RA + CCR7-) human TEMRA from PBMCs of control ( n = 10) and individuals with Alstrom syndrome ( n = 10). Data are presented as mean +/− SD. Paired two-tailed Wilcoxon T test; * P < 0.05. ( E ) Scatter plots showing the percentage of human CD4 TEMRA (CCR7-CD45RO-) cells from PBMCs from individuals ( n = 13) before and after semaglutide treatment. All samples were gated on live (near IR-) populations. Paired nonparametric Wilcoxon T test; n.s. ( F ) Flow cytometry analysis of human CD4 + TEMRA cells (CD3 + CD4 + CD45RA + CD27 − ) in subcutaneous abdominal adipose tissue and lysed whole blood. In adipose, data are expressed as a percentage of CD4 + T cells. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention= 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s. ( G ) Heatmap displaying transcriptomic data of selected genes with previous implications with autophagy and/or senescence from murine CD44+ memory T cells isolated from spleen of female C57BL/6J mice on different diet groups for 14-week ( n = 3 female C57BL/6J mice per group; groups 1–3 in Fig. ). The analysis was performed using the Nanostring Mouse PanCancer Immune Profiling panel, covering 770 genes including genes related to autophagy and/or senescence. .

    Journal: EMBO Reports

    Article Title: DNA methylation-mediated memory of obesity in CD4 T lymphocytes perpetuates immune dysregulation

    doi: 10.1038/s44319-026-00765-w

    Figure Lengend Snippet: ( A ) Representative western blots (top) and densitometric quantification (bottom) of Stk26 protein levels within peripheral LNs CD4 T cells isolated from female C57BL/6J mice on different diet groups for 14 weeks (groups 1–3 in Fig. ). Data are presented as mean +/− SD ( n = 3 mice per group). Unpaired nonparametric Mann–Whitney T test; n.s. ( B ) Representative flow cytometry histograms (left) showing LC3II expression in splenocytes from the three 14-week mouse groups (groups 1–3 in Fig. ). Splenocytes were cultured ex vivo in the presence of 100 μM of chloroquine within RPMI for 4 h. Scatter plots (right) show autophagy flux, calculated as the difference in mean fluorescence intensity (MFI) of LC3II between chloroquine-treated and vehicle control groups. Analysis was gated on live (near IR-) CD4 + CD44 + CXCR3+ or CD4 + CD44 + LFA1 + T cell populations. Data are from one experiment ( n = 6 female C57BL/6J mice per group). Results are shown as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), ** P < 0.01. ( C ) Representative flow cytometry plots (left) of CD4 + KLRG1+ cells gated within the live (near IR-) CD4 T cell population from peripheral LNs of female C57BL/6J mice on a 14-week CD or HFD-RE. Scatter plots (right) show the percentage of CD4 + KLRG1+ cells within the CD4 + CD44+ or CD4 + LFA1+ populations. Data is from one experiment ( n = 5–6 mice per group) and is presented as mean +/− SD. Unpaired nonparametric T test (Mann–Whitney), * P < 0.05. ( D ) Scatter plots showing CD4 and CD8 (CD45RA + CCR7-) human TEMRA from PBMCs of control ( n = 10) and individuals with Alstrom syndrome ( n = 10). Data are presented as mean +/− SD. Paired two-tailed Wilcoxon T test; * P < 0.05. ( E ) Scatter plots showing the percentage of human CD4 TEMRA (CCR7-CD45RO-) cells from PBMCs from individuals ( n = 13) before and after semaglutide treatment. All samples were gated on live (near IR-) populations. Paired nonparametric Wilcoxon T test; n.s. ( F ) Flow cytometry analysis of human CD4 + TEMRA cells (CD3 + CD4 + CD45RA + CD27 − ) in subcutaneous abdominal adipose tissue and lysed whole blood. In adipose, data are expressed as a percentage of CD4 + T cells. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention= 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s. ( G ) Heatmap displaying transcriptomic data of selected genes with previous implications with autophagy and/or senescence from murine CD44+ memory T cells isolated from spleen of female C57BL/6J mice on different diet groups for 14-week ( n = 3 female C57BL/6J mice per group; groups 1–3 in Fig. ). The analysis was performed using the Nanostring Mouse PanCancer Immune Profiling panel, covering 770 genes including genes related to autophagy and/or senescence. .

    Article Snippet: Human CD45RA (clone: REA562) , Miltenyi Biotech , Cat #130-113-363.

    Techniques: Western Blot, Isolation, MANN-WHITNEY, Flow Cytometry, Expressing, Cell Culture, Ex Vivo, Fluorescence, Control, Two Tailed Test

    ( A ) Scatter box plots showing flow cytometry analysis of the percentage of human CD4 and CD8 Tem (CD45RA-CCR7-) Tem cells from PBMCs isolated from control individuals ( n = 10) or individuals with Alstrom syndrome ( n = 10). Data are presented as mean +/− SD. Unpaired nonparametric Mann–Whitney T test; * P < 0.05. ( B , C ) Flow cytometry analysis of subcutaneous human abdominal adipose tissue ( B ) and lysed whole blood ( C ), including CD8 + T cells, and the CD8 + T cell sub-populations; naive (CD3 + CD8 + CD45RA + CD27 + ), central memory (CD3 + CD8 + CD45RA − CD27 + ), effector memory (CD3 + CD8 + CD45RA − CD27 − ). In adipose, CD8 + T cells are expressed as a percentage of CD3+ events, and CD8 + T cell sub-populations are expressed as a percentage of CD8+ events. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to a control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention = 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s.

    Journal: EMBO Reports

    Article Title: DNA methylation-mediated memory of obesity in CD4 T lymphocytes perpetuates immune dysregulation

    doi: 10.1038/s44319-026-00765-w

    Figure Lengend Snippet: ( A ) Scatter box plots showing flow cytometry analysis of the percentage of human CD4 and CD8 Tem (CD45RA-CCR7-) Tem cells from PBMCs isolated from control individuals ( n = 10) or individuals with Alstrom syndrome ( n = 10). Data are presented as mean +/− SD. Unpaired nonparametric Mann–Whitney T test; * P < 0.05. ( B , C ) Flow cytometry analysis of subcutaneous human abdominal adipose tissue ( B ) and lysed whole blood ( C ), including CD8 + T cells, and the CD8 + T cell sub-populations; naive (CD3 + CD8 + CD45RA + CD27 + ), central memory (CD3 + CD8 + CD45RA − CD27 + ), effector memory (CD3 + CD8 + CD45RA − CD27 − ). In adipose, CD8 + T cells are expressed as a percentage of CD3+ events, and CD8 + T cell sub-populations are expressed as a percentage of CD8+ events. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to a control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention = 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s.

    Article Snippet: Human CD45RA (clone: REA562) , Miltenyi Biotech , Cat #130-113-363.

    Techniques: Flow Cytometry, Isolation, Control, MANN-WHITNEY

    ( A ) Scatter plots showing flow cytometry analysis of the percentage of human CD4 T cells, CD4 Tem (CCR7-CD45RO + ) and inflammatory CD4 T cells (CXCR3 + ) from PBMCs isolated from individuals ( n = 13) before and after semaglutide treatment. All samples were gated on live populations. Paired nonparametric Wilcoxon T test; n.s. ( B , C ) Flow cytometry analysis of human subcutaneous abdominal adipose tissue ( B ) and lysed whole blood ( C ), including CD3 + T cells, CD4 + T cells, and the CD4 + T cell sub-populations; naive (CD3 + CD4 + CD45RA + CD27 + ), central memory (CD3 + CD4 + CD45RA-CD27 + ), effector memory (CD3 + CD4 + CD45RA − CD27 − ). In adipose, CD3 + T cells are expressed as a percentage of CD45+ events, CD4 + T cells are expressed as a percentage of CD3+ events, and CD4 + T cell sub-populations are expressed as a percentage of CD4+ events. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to a control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention = 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s. .

    Journal: EMBO Reports

    Article Title: DNA methylation-mediated memory of obesity in CD4 T lymphocytes perpetuates immune dysregulation

    doi: 10.1038/s44319-026-00765-w

    Figure Lengend Snippet: ( A ) Scatter plots showing flow cytometry analysis of the percentage of human CD4 T cells, CD4 Tem (CCR7-CD45RO + ) and inflammatory CD4 T cells (CXCR3 + ) from PBMCs isolated from individuals ( n = 13) before and after semaglutide treatment. All samples were gated on live populations. Paired nonparametric Wilcoxon T test; n.s. ( B , C ) Flow cytometry analysis of human subcutaneous abdominal adipose tissue ( B ) and lysed whole blood ( C ), including CD3 + T cells, CD4 + T cells, and the CD4 + T cell sub-populations; naive (CD3 + CD4 + CD45RA + CD27 + ), central memory (CD3 + CD4 + CD45RA-CD27 + ), effector memory (CD3 + CD4 + CD45RA − CD27 − ). In adipose, CD3 + T cells are expressed as a percentage of CD45+ events, CD4 + T cells are expressed as a percentage of CD3+ events, and CD4 + T cell sub-populations are expressed as a percentage of CD4+ events. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to a control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention = 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s. .

    Article Snippet: Human CD45RA (clone: REA562) , Miltenyi Biotech , Cat #130-113-363.

    Techniques: Flow Cytometry, Isolation, Control

    ( A ) Bar graph showing the estimated protein copy numbers of STK26 in human CD4 T cell subsets, derived from the ImmPres dataset ( http://immpres.co.uk/ ). ( B ) Bar graph showing the estimated protein copy numbers of STK26 in human CD8 T cell subsets, derived from the ImmPres dataset ( http://immpres.co.uk/ ). ( C ) Flow cytometry analysis of human subcutaneous abdominal adipose tissue and lysed whole blood, including CD8 + TEMRA cells (CD3 + CD8 + CD45RA + CD27 − ). In adipose, data are expressed as a percentage of CD8 + T cells. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to a control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention = 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s.

    Journal: EMBO Reports

    Article Title: DNA methylation-mediated memory of obesity in CD4 T lymphocytes perpetuates immune dysregulation

    doi: 10.1038/s44319-026-00765-w

    Figure Lengend Snippet: ( A ) Bar graph showing the estimated protein copy numbers of STK26 in human CD4 T cell subsets, derived from the ImmPres dataset ( http://immpres.co.uk/ ). ( B ) Bar graph showing the estimated protein copy numbers of STK26 in human CD8 T cell subsets, derived from the ImmPres dataset ( http://immpres.co.uk/ ). ( C ) Flow cytometry analysis of human subcutaneous abdominal adipose tissue and lysed whole blood, including CD8 + TEMRA cells (CD3 + CD8 + CD45RA + CD27 − ). In adipose, data are expressed as a percentage of CD8 + T cells. Samples are from participants of a 10-week randomized controlled trial of exercise training ( n = 14) compared to a control ( n = 13). Pre-intervention = day one of the intervention (before exercise). Post-intervention = 36 h after the 10-week intervention/control period. Repeated measures analyses of variance (ANOVAs); n.s.

    Article Snippet: Human CD45RA (clone: REA562) , Miltenyi Biotech , Cat #130-113-363.

    Techniques: Derivative Assay, Flow Cytometry, Control